b) Monotrichous d) Malaria A) True View Answer, 35. Acridine orange is which type of mutagen? View Answer, 18. 5. a) viruses Everything you need to know about water and wastewater microbiology. 4.1: Prokaryote Habitats, Relationships, and Microbiomes. 8. Microorganisms are minute, unicellular organisms that aren't visible to the naked eye. d) The organism must be able to grow in an easily available nutrient medium Which of the following is a correct usage of binomial nomenclature? Which of the following pulmonary pathogens cannot be easily detected by the gram staining technique when performing a microscopy test? The microbes play a major role in nutrient cycling, decomposition, food spoilage, control and cause of diseases and biotechnology. It is important to find and resolve the source of the contamination. (retrieve https://www.learningsta.com, April 2021) 2.0 SCREENING . 9. c) The optimum temperature for the growth of an organism must be above 50C. 2011-2023 Sanfoundry. Answer: B 31) Name the bacteria of the Enterobacteriaceae family that have the following characteristics? ticks and fleas. 1. View Answer, 7. View Answer, 48. Sulphur can be utilized by bacteria in the a. a) Alcoholic beverages Which of the following method is useful for detection and isolation of those microorganisms which are capable of growing on a particular nutrient medium? Most E. coli bacteria are harmless and are found in great quantities in the intestines of people and warm-blooded animals. The section contains questions and answers on sanitary quality of water, wastewater, treatment processes, fresh foods microbial flora, food preservation and microorganisms. Answer: C Performance and sterility testing of media lots should also be determined and B. Auxanographic technique Which of the following enzyme removes the RNA primer with its 5-nuclease activity? b) Automatic Type Counter & Classifier Universal aseptic techniques includes, Except a) Gown b) Mask c) Cough Etiquettes d) Hand washing answer-D 4. c) Saccharomyces cerevisiae Which of the following method is useful for the isolation and detection of organisms having the ability to produce growth factors? Our 1000+ MCQs focus on all topics of the Microbiology subject, covering 100+ topics. 5. View Answer, 23. The OpenStax name, OpenStax logo, OpenStax book covers, OpenStax CNX name, and OpenStax CNX logo c) Enrichment Culture technique Microscopic examination and culture of the sputum sample revealed gram-positive cocci that appear in chains. includes 6 urine samples. b) Thymol blue In modern plants the screens are cleaned . determined to obtain accurate susceptibility results. Pathogen testing is used to identify pathogenic organisms in manufacturing environments, ingredients or finished products that could harm the consumer. c) Phenolphthalein Gram positive bacterial cell wall takes the stain of crystal violet and appears, BIOCHEMISTRY, BIOMEDICINE & PHARMACEUTICS, Classification and identification of microorganisms, Branched Chain Amino Acid Metabolism and Maple Syrup Urine Disease, drugs for the treatment of cardiovascular diseases, MEDICAL MICROBIOLOGY gastrointestinal tract, Nosocomial infection/ hospital acquired infections, Lipid Metabolism: MCQs (Cholesterol, Triglycerides, and Other Lipids), MCQs on Sterilization and Disinfection - Decontamination, aseptic processes in laboratory and hospitals, Carbohydrate Metabolism: MCQ on Glycolysis & Gluconeogenesis. What is required for proper isolation of microorganisms? D. American Type Classifier and Collection. As primary screening allows the detection and isolation of microorganisms which posses, potentially interesting industrial applications. d) Coliform bacteria. Official Website of Dr. Shyama Prasad Mukherjee University, Ranchi . Cryptococcosis is a disease of ________________ 2. Who is considered the "father of Western medicine"? The greatest resolution in light microscopy can be obtained with ___________ The principal microorganism for yogurt is ______________________ 42) Which of the following are the routine diagnostic methods performed for the microbial examination of blood specimens collected from a patient with a high fever for more than 2 days? d) Amino acid producers a) above 1 percent If you are redistributing all or part of this book in a print format, Which among the following group of microorganisms are found in the ducts of cows mammary glands? c) Organic acid producers c) Streptococcus thermophilus d. using damaged equipment and glassware. Juli 2022 The cell structure of gram positive and gram negative bacteria appear as the picture given below after the gram staining procedure, Gram positive bacteria Gram negative bacteria. c) carbohydrates b) False All the microorganisms such as amoeba, protozoa, viruses, bacteria are studied under microbiology. Q. Vegetative bacteria are able to withstand adverse conditions better than protozoan cysts. Which of the following shows the zone of inhibition when a particular organism is grown on a Petri plate? D. Indicator dye technique. 7. A. c) 2.5 to 4 percent Microbes can live in highly acidic as well as an alkaline medium. B) False, Yeast cells prefer aerobic conditions to multiply in number. The microorganisms from lakes and rivers can grow at a salt concentration of _________ View Answer, 49. Which of the following method is useful for isolation and detection of organisms having the ability to produce organic acids? Glycolysis can occur in ___________ Which scientist proposed adding a kingdom for protists? Full-form of ATCC is _________ d) Fungi d) Indicator dye technique Clarification: The transfer of a 1ml solution to 9ml d/w is called as serial 10-fold dilution. Crowded plate technique is used for screening of____________. c) rod shaped Primary Screening of Microorganisms: Primary screening may be defined as detection and isolation of the desired microorganism based on its qualitative ability to produce the desired product like antibiotic or amino acid or an enzyme etc. B. Auxanographic technique Which among the following is a pathogenic algae for humans? The section contains multiple choice questions and answers on soil microorganisms interactions, nitrogen, carbon and sulphur cycles, aquatic environment, aquatic microorganisms techniques and productivity. It includes the physical processes of screening, comminution, grit removal, and sedimentation. For which viral disease, vaccine has been recently developed through the use of tissue culture? 6. 8. View Answer, 3. Home Fermentation Technology Objective Questions 250+ MCQs on Isolation and Screening of Industrially Important Microbes and Answers. B) False, Hot foods must be maintained above 60 oC to inhibit microbial growth. Which part of the compound microscope helps in gathering and focusing light rays on the specimen to be viewed? View Answer, 45. d) Study of microscope The section contains multiple choice questions and answers on microorganisms characteristics and microbial classification. Which of the following statements about food chain and energy . A) manipulating the growth environment. View Answer, 14. 2. Secondary screening can give information about, (1) The genetic instability in microbial cultures, (2) Number of products produced in a single fermentation, 8. 9. b) light waves It will immensely help anyone trying to crack an exam or an interview. If you would like to learn "Microbiology" thoroughly, you should attempt to work on the complete set of 1000+ MCQs - multiple choice questions and answers mentioned above. B) sulfites. Eubacteria can be either gram-negative or gram-positive, they have economic, agricultural, and medical importance. B. Crowded plate technique Therefore, the exact composition of a defined medium is known. a) Methyl red a) Crowded plate technique d) Indicator dye technique D) all of the above. The well-isolated colonies are antibiotic producers with the zone of inhibition. View Answer, 44. Which of the following is NOT a domain in Woese and Foxs phylogenetic tree? Which of the following method is useful for the isolation and detection of organisms having the ability to produce antibiotics? 3. B) are by-products of metabolism that are not important to microbe function. There are various microorganisms types like bacteria, fungi, archaea, or protists. (3) It does not provide many ideas about the production or yield potential of microorganisms. Which of the following is the source of Vitamin A. b) Nitrosococcus oceanus (1) Optimal temperature for growth, (2) Optimal oxygen requirements, (3) Optimal nutritional needs (4) All of the above. 11. (Or show it to your microbiology professor, just for fun.) D. Rose Bengal Agar plate. Which of the following method is useful for isolation and detection of organisms having the ability to produce organic acids? Which shape of bacteria has been represented in the following diagram?a) Rod-shapedb) Comma shapedc) Spiral shapedd) Spherical shapedAnswer: aClarification: The following diagram represents rod-shaped bacteria, magnified 1500X. How do you isolate microorganisms from the soil? It belongs to the phthalein family that is employed as an acid-base indicator. a) Shortest wavelength of visible light used The bacterial cell then elongates and splits into two daughter cells each with identical DNA to the parent cell. False. a) cytoplasmic membrane 15. Which of the following method is not used in isolation and screening of desired microorganisms? The screening is isolation and detection of microorganisms of interest. b) Antibiotic producers Answer: B Has two nuclei C. Forms cysts D. Has a sucking disc E. Survives well in ordinary water, even when this is hot or contains chlorine or detergents, Flagellate parasite is the cause of vulvovaginitis in women in . Label these plates 1/10 3 through 1/10 6. b) aerobic cells 3. Fecal coliform bacteria are a subgroup of total coliform bacteria. chipotle corporate phone number . Screening includes primary screening and secondary screening. Types of pathogens include specific bacteria strains, viruses, fungi, or parasites that can cause illness in its host. D. Indicator dye technique. It is further followed by secondary screening, to check the capabilities and gain information about these organisms. c) Mumps Which of the following are produced by microorganisms? Answer: B (2) It determines which microorganisms can produce a compound. 5. 6. Some examples of rod-shaped bacteria include Lactobacillus, Morganella, Bacillus anthracis, Klebsiella pneumoniae. 1. The serial dilutions of soil are performed and spread on a Petri plate and allowed to grow. Secondary metabolites C) use of microbes in sewage and pollution control. In the following diagram, what does the question mark represent?a) Ciliab) Flagellac) Haird) PseudopodiaAnswer: b Secondary screening may be qualitative and quantitative in its approach. Fermentation Technology Questions on Isolation and Screening of Industrially Important Microbes. d) Shortest wavelength of visible light used and an objective with the maximum numerical aperture (4) This may be accomplished by paper, thin layer, chromatographic technique. a) 1mm in diameter Answer: C coliform bacteria in drinking water, the source is probably environmental and fecal contamination is unlikely. Textbook content produced by OpenStax is licensed under a Creative Commons Attribution License . c) Cyanobacteria 9. d) 2 micrometer in diameter Microbiology is the study of living organisms that are not visible to the naked eyes. Which of the following method is not used in isolation and screening of desired microorganisms? a) Bacillariophycophyta Several microbes are useful to humans in diverse ways like in household products, industrial products, agricultural products, etc. E. coli is a sub-group of the fecal coliform group. d) Phaeophycophyta c) Study of organisms that are not visible to naked eyes 3. Who was the first to observe "animalcules" under the microscope? d) electron beams a) True 1999-2023, Rice University. Clarification: The Antibiotic producers show the zone of inhibition. View Answer, 41. a) Treponema Which of the following gram positive bacteria is a strict anaerobe and can only grow in the absence of oxygen? other viruses are too dangerous to work with in a clinical lab setting. A) True Required fields are marked *. 2011-2023 Sanfoundry. 1. Our mission is to improve educational access and learning for everyone. Clarification: The Crowded Plate Technique is used for the detection and isolation of the organisms which are antibiotic producers. View Answer, 39. c) Enrichment Culture technique d) Indicator dye technique 26. Answer: A Each daughter cell is a clone of the parent cell. b) below 1 percent c) MacConkeys Agar plate View Answer, 43. C) are products that require additional processing before they can be packaged. B) 62.3oC for 30 minutes. MCQ Questions for Class 8 Science: Ch 2 Microorganisms: Friend and Foe. Through primary screening only few or many microorganism that produce a industrially . Lipopolysaccharide in cell walls is characteristic of? 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It gets its name from the Danish bacteriologist Hans Christian Gram who first introduced it in 1882, mainly to identify organisms causing pneumonia. Clarification: Phenol Red plate is used to detect and isolate organic acid producers. 7. D. Phenol red. c) 1000-fold For this dilution, it may be said that the solution was diluted _______ A 70 year old male visited a clinic with chronic cough, chest pain, fever, chills, and weakness. The section contains multiple choice questions and answers on morphology, reproduction, physiology and fungi cultivation, fungi classification and special interest. Clarification: The prototrophs are the organisms which are capable of synthesizing all the growth requirements by themselves whereas auxotroph is dependent on prototrophs. Examples of microorganisms include bacteria, archaea, algae, protozoa, and microscopic animals such as the dust mite. Set up a series of 1/10 dilutions: a. c) Spirochetes The section contains MCQs on diseases caused by fungi and protozoa, therapeutic drugs for fungal treatment and protozoan diseases. You can also prevent infections through: Hand-washing. Viral hemagglutination assays only work with certain types of viruses because. B. a) anaerobic cells Includes common viruses, bacteria, and parasites 1,2: Stool sample: Sensitive, certain positive results may be unrelated to active infection. c) cytoplasm (1) Screen organic acid-producing microbes. b) Killing pathogenic microorganisms c) Killing spores d) None of these answer-A 3. a) Cephaleuros This will help you to prepare for exams, contests, online tests, quizzes, viva-voce, interviews, and certifications. Antibiotic producers b. not washing hands before and after lab . Indeed, until Anton von Leeuwenhoek invented . c) Streptococci. Microorganisms Class 8 Science Chapter 2 as per NCERT Book used in CBSE and other Schools. Now, 1ml of that stock solution is taken and transferred to the 2nd test tube containing 9ml of distilled water. True Q.1. 1g of soil is added to that test tube and the soil is allowed to settle down. Which of the following is used in electron microscope? View Answer, 24. b) mutualistic View Answer, 2. d) Lophotrichous C) selecting microbial strains that lack a feedback system. (2) Chemical, physical and biological properties of a product are also determined during secondary screening. Some examples of rod-shaped bacteria include Lactobacillus, Morganella, Bacillus anthracis, Klebsiella . Pathogen testing is continuously performed throughout the production . 3. Which of the following is not a subfield of microbiology? 2. 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View Answer. a) Styloviridae A 24 year old sexually active woman had an infection in the vaginal area with genital discharge while urinating. Prokaryotes can be found everywhere on our planet, even in the most extreme environments. Prokaryotes are unicellular microorganisms whose cells have no nucleus. In which of the following treatment involve oxidation of organic constituents of the wastewater? c) UV light a) 3.0 4.6 B. Auxanographic technique Wash your hands with soap and water for at least 20 seconds. a) viral infection The common symptoms are pink or red spots on the skin surface, dry cough, and runny nose. View Answer, 42. A skin biopsy test was performed and the infection was diagnosed to be a parasitic infection. b) gamma rays a) x-rays He came with a lesion on the lower part of the left leg that according to him first appeared about a week before visiting the hospital. View Answer, 8. True Bacterial cell grown on hydrocarbon wastes from the petroleum industry are a source of _____________ D) are harvested during the exponential phase of growth. A food chain comprising birds, green plants, fish and man. A. (1) It is useful in sorting microorganisms that have real commercial value. Secondary screening helps in detecting really useful microorganisms in fermentation processes, which includes 10. c) chitin c) Spirillum volutans 4. Which of the following method proceeds with 2-plate preparation? Clarification: The optimum temperature for the growth of an organism should be above 40C. uses 100 grams of glucose in a 300ml solution. Which of the following microorganism is eliminated in canned foods? 5. 5. Clarification: The Enrichment culture technique is useful for the detection and isolation of those microorganisms which are capable of growing on a particular nutrient medium. A. The presence of E. coli in a drinking water sample almost always indicates recent fecal contamination, meaning there is a greater . A. American Type Culture Collection d) intercalating agents d) appendaged bacteria a) Phenol red plate 5. The process is repeated several times until the requirement is met. The microorganisms are chosen based on the rest three criteria. 6. Self-Assessment Quiz. The Microbiology Society has a vision and mission around which we base our strategy. d) Hanging Drop technique Q. Bacterial endospores are more resistant to antimicrobial control methods than all other microbial forms. Answer: 4. B. Which of the following method is useful for the detection and isolation of those microorganisms which are capable of growing on a particular nutrient medium? C. Enrichment Culture technique View Answer, 46. a) S mallpox b) cell wall Full-form of ATCC is _________. The lesson covers the complete explanation of class 8 Chapter 2 Microorganisms.Topics covered are Introduction to Micro-organisms and microbiology, different types of Micro-organisms, Useful and Harmful Micro-organisms. lauren conrad and stephen colletti /; 2. 43) Which of the following methods are the most common and routine culture methods performed in the microbiology lab for the proper isolation and identification of bacteria and fungi? a) False a) Lactobacillus c) holozoic C) alcohol. The section contains questions and answers on diseases caused by staphylococcus, streptococcus, corynebacterium, aerobic and anaerobic gram, viruses containing single and double stranded dna. Clarification: The Auxanographic technique is used for the detection and isolation of the organisms which are growth factor producers. Crowded plate technique Report an issue. View Answer, 5. d) Eyepiece lens d) Nitrosovibrio tenuis C) is used to prevent growth of halophiles. d) Measles Which one of the following organisms is least likely to cause this illness? B) False, The aim of industrial microbiology is to produce chemicals that can be purified and packaged for sale or used in other commercial processes. 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B) False, Microbiology 17 immunization and immune Testi. The reason they are called microorganisms is that you can only see them when you use a microscope. Secondary screening can give information about (1) The genetic instability in microbial cultures (2) Number of products produced in a single fermentation (3) The structure of the product (4) All of the above. (1) Its a process for the detection and isolation of microorganisms of our interest. Automatic Type Counter & Classifier Avoid contaminated food and water. 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